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Analyses of Very Early Hemopoietic Regeneration After Bone Marrow Transplantation: Comparison of Intravenous and Intrabone Marrow Routes

In bone marrow transplantation (BMT), bone marrow cells (BMCs) have traditionally been injected intravenously. However, remarkable advantages of BMT via the intra‐bone‐marrow (IBM) route (IBM‐BMT) over the intravenous route (IV‐BMT) have been recently documented by several laboratories. To clarify t... Full description

Journal Title: STEM CELLS May 2007, Vol.25(5), pp.1186-1194
Main Author: Li, Qing
Other Authors: Hisha, Hiroko , Yasumizu, Ryoji , Fan, Tian‐Xue , Yang, Guo‐Xiang , Li, Qiang , Cui, Yun‐Ze , Wang, Xiao‐Li , Song, Chang‐Ye , Okazaki, Satoshi , Mizokami, Tomomi , Cui, Wen‐Hao , Guo, Kequan , Li, Ming , Feng, Wei , Katou, Junko , Ikehara, Susumu
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1066-5099 ; E-ISSN: 1549-4918 ; DOI: 10.1634/stemcells.2006-0354
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recordid: wj10.1634/stemcells.2006-0354
title: Analyses of Very Early Hemopoietic Regeneration After Bone Marrow Transplantation: Comparison of Intravenous and Intrabone Marrow Routes
format: Article
creator:
  • Li, Qing
  • Hisha, Hiroko
  • Yasumizu, Ryoji
  • Fan, Tian‐Xue
  • Yang, Guo‐Xiang
  • Li, Qiang
  • Cui, Yun‐Ze
  • Wang, Xiao‐Li
  • Song, Chang‐Ye
  • Okazaki, Satoshi
  • Mizokami, Tomomi
  • Cui, Wen‐Hao
  • Guo, Kequan
  • Li, Ming
  • Feng, Wei
  • Katou, Junko
  • Ikehara, Susumu
subjects:
  • Intravenous‐Bone Marrow Transplantation
  • Intrabone Marrow‐Bone Marrow Transplantation
  • Hemopoietic Regeneration Chemotaxis
ispartof: STEM CELLS, May 2007, Vol.25(5), pp.1186-1194
description: In bone marrow transplantation (BMT), bone marrow cells (BMCs) have traditionally been injected intravenously. However, remarkable advantages of BMT via the intra‐bone‐marrow (IBM) route (IBM‐BMT) over the intravenous route (IV‐BMT) have been recently documented by several laboratories. To clarify the mechanisms underlying these advantages, we analyzed the kinetics of hemopoietic regeneration after IBM‐BMT or IV‐BMT in normal strains of mice. At the site of the direct injection of BMCs, significantly higher numbers of donor‐derived cells in total and of c‐kit cells were observed at 2 through 6 days after IBM‐BMT. In parallel, significantly higher numbers of colony‐forming units in spleen were obtained from the site of BMC injection. During this early period, higher accumulations of both hemopoietic cells and stromal cells were observed at the site of BMC injection by the IBM‐BMT route. The production of chemotactic factors, which can promote the migration of a BM stromal cell line, was observed in BMCs obtained from irradiated mice as early as 4 hours after irradiation, and the production lasted for at least 4 days. In contrast, sera collected from the irradiated mice showed no chemotactic activity, indicating that donor BM stromal cells that entered systemic circulation cannot home effectively into recipient bone cavity. These results strongly suggest that the concomitant regeneration of microenvironmental and hemopoietic compartments in the marrow (direct interaction between them at the site of injection) contributes to the advantages of IBM‐BMT over IV‐BMT. Disclosure of potential conflicts of interest is found at the end of this article.
language: eng
source:
identifier: ISSN: 1066-5099 ; E-ISSN: 1549-4918 ; DOI: 10.1634/stemcells.2006-0354
fulltext: fulltext
issn:
  • 1066-5099
  • 10665099
  • 1549-4918
  • 15494918
url: Link


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titleAnalyses of Very Early Hemopoietic Regeneration After Bone Marrow Transplantation: Comparison of Intravenous and Intrabone Marrow Routes
creatorLi, Qing ; Hisha, Hiroko ; Yasumizu, Ryoji ; Fan, Tian‐Xue ; Yang, Guo‐Xiang ; Li, Qiang ; Cui, Yun‐Ze ; Wang, Xiao‐Li ; Song, Chang‐Ye ; Okazaki, Satoshi ; Mizokami, Tomomi ; Cui, Wen‐Hao ; Guo, Kequan ; Li, Ming ; Feng, Wei ; Katou, Junko ; Ikehara, Susumu
ispartofSTEM CELLS, May 2007, Vol.25(5), pp.1186-1194
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subjectIntravenous‐Bone Marrow Transplantation ; Intrabone Marrow‐Bone Marrow Transplantation ; Hemopoietic Regeneration Chemotaxis
descriptionIn bone marrow transplantation (BMT), bone marrow cells (BMCs) have traditionally been injected intravenously. However, remarkable advantages of BMT via the intra‐bone‐marrow (IBM) route (IBM‐BMT) over the intravenous route (IV‐BMT) have been recently documented by several laboratories. To clarify the mechanisms underlying these advantages, we analyzed the kinetics of hemopoietic regeneration after IBM‐BMT or IV‐BMT in normal strains of mice. At the site of the direct injection of BMCs, significantly higher numbers of donor‐derived cells in total and of c‐kit cells were observed at 2 through 6 days after IBM‐BMT. In parallel, significantly higher numbers of colony‐forming units in spleen were obtained from the site of BMC injection. During this early period, higher accumulations of both hemopoietic cells and stromal cells were observed at the site of BMC injection by the IBM‐BMT route. The production of chemotactic factors, which can promote the migration of a BM stromal cell line, was observed in BMCs obtained from irradiated mice as early as 4 hours after irradiation, and the production lasted for at least 4 days. In contrast, sera collected from the irradiated mice showed no chemotactic activity, indicating that donor BM stromal cells that entered systemic circulation cannot home effectively into recipient bone cavity. These results strongly suggest that the concomitant regeneration of microenvironmental and hemopoietic compartments in the marrow (direct interaction between them at the site of injection) contributes to the advantages of IBM‐BMT over IV‐BMT. Disclosure of potential conflicts of interest is found at the end of this article.
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titleAnalyses of Very Early Hemopoietic Regeneration After Bone Marrow Transplantation: Comparison of Intravenous and Intrabone Marrow Routes
descriptionIn bone marrow transplantation (BMT), bone marrow cells (BMCs) have traditionally been injected intravenously. However, remarkable advantages of BMT via the intra‐bone‐marrow (IBM) route (IBM‐BMT) over the intravenous route (IV‐BMT) have been recently documented by several laboratories. To clarify the mechanisms underlying these advantages, we analyzed the kinetics of hemopoietic regeneration after IBM‐BMT or IV‐BMT in normal strains of mice. At the site of the direct injection of BMCs, significantly higher numbers of donor‐derived cells in total and of c‐kit cells were observed at 2 through 6 days after IBM‐BMT. In parallel, significantly higher numbers of colony‐forming units in spleen were obtained from the site of BMC injection. During this early period, higher accumulations of both hemopoietic cells and stromal cells were observed at the site of BMC injection by the IBM‐BMT route. The production of chemotactic factors, which can promote the migration of a BM stromal cell line, was observed in BMCs obtained from irradiated mice as early as 4 hours after irradiation, and the production lasted for at least 4 days. In contrast, sera collected from the irradiated mice showed no chemotactic activity, indicating that donor BM stromal cells that entered systemic circulation cannot home effectively into recipient bone cavity. These results strongly suggest that the concomitant regeneration of microenvironmental and hemopoietic compartments in the marrow (direct interaction between them at the site of injection) contributes to the advantages of IBM‐BMT over IV‐BMT. Disclosure of potential conflicts of interest is found at the end of this article.
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abstractIn bone marrow transplantation (BMT), bone marrow cells (BMCs) have traditionally been injected intravenously. However, remarkable advantages of BMT via the intra‐bone‐marrow (IBM) route (IBM‐BMT) over the intravenous route (IV‐BMT) have been recently documented by several laboratories. To clarify the mechanisms underlying these advantages, we analyzed the kinetics of hemopoietic regeneration after IBM‐BMT or IV‐BMT in normal strains of mice. At the site of the direct injection of BMCs, significantly higher numbers of donor‐derived cells in total and of c‐kit cells were observed at 2 through 6 days after IBM‐BMT. In parallel, significantly higher numbers of colony‐forming units in spleen were obtained from the site of BMC injection. During this early period, higher accumulations of both hemopoietic cells and stromal cells were observed at the site of BMC injection by the IBM‐BMT route. The production of chemotactic factors, which can promote the migration of a BM stromal cell line, was observed in BMCs obtained from irradiated mice as early as 4 hours after irradiation, and the production lasted for at least 4 days. In contrast, sera collected from the irradiated mice showed no chemotactic activity, indicating that donor BM stromal cells that entered systemic circulation cannot home effectively into recipient bone cavity. These results strongly suggest that the concomitant regeneration of microenvironmental and hemopoietic compartments in the marrow (direct interaction between them at the site of injection) contributes to the advantages of IBM‐BMT over IV‐BMT. Disclosure of potential conflicts of interest is found at the end of this article.
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