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Molecular analysis of genes acting in fruiting body development in basidiomycetes / by Prayook Srivilai

Coprinopsis cinerea is an excellent model for studying fruiting body development in the higher basidiomycetes because it has a short life cycle, grows and fruits on artificial media under laboratory conditions, and in addition its genome has been released. This thesis is focused on the action of gen... Full description

PPN (Catalogue-ID): 561371008
Personen: Srivilai, Prayook [VerfasserIn]
Format: eBook eBook
Language: English
Sprache der Zusammenfassung: Englisch
Published: Göttingen, Niedersächsische Staats- und Universitätsbibliothek,
Hochschule: @Göttingen, Univ., Diss., 2006
Basisklassifikation: 42.13 Molekularbiologie
42.38 Botanik: Allgemeines
58.30 Biotechnologie
Subjects:

Ständerpilze

Fruchtkörperbildung

Genaktivität

Molekularbiologie

Analyse

Formangabe: Hochschulschrift
Physical Description: Online Ressource (241 S., 231.262 KB), Ill., graph. Darst.

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520 |a Coprinopsis cinerea is an excellent model for studying fruiting body development in the higher basidiomycetes because it has a short life cycle, grows and fruits on artificial media under laboratory conditions, and in addition its genome has been released. This thesis is focused on the action of genes in fruiting body development in C. cinerea. Sexual development is regulated by the A and B mating type genes that encodes two types of homeodomain transcription factors (called HD1 and HD2) and pheromones and pheromone receptors, respectively. Self-fertile strains with defects in the mating type genes such as homokaryon AmutBmut are used to create mutants in the fruiting pathway. Co-isogenic strains with different wild type mating type specificities have been constructed from homokaryon AmutBmut by repetitive backcrosses. The quality of the obtained co-isogenic strains was tested by molecular RAPD (Randomly Amplification Polymorphism DNA) markers. A co-isogenic strain has been used to follow up in a genetic cross mutant genes in fruiting body development. In this way, three different mutations (cfs1, mat and bad) in the AmutBmut UV-mutant 6-031 could be separated. Gene cfs1 for a cyclopropane fatty acid synthase acts in the initiation of fruiting body development. The other mutations are in a gene for fruiting body primordia maturation (mat) and in a gene for basidiospore production (bad). Strain OU3-1 containing the cfs1 mutation and the defective mating type genes served further in analyzing the essential cfs1 function in fruiting development by transformation with the cloned wild type gene ... 
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