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Glycobiology / ed. by Minoru Fukuda

In this 3 volume collection focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers' own research efforts Each chapter has been designed so that enough scientific background will be given in each chapter for further development of... Full description

PPN (Catalogue-ID): 644586494
Personen: Fukuda, Minoru
Format: eBook eBook
Enthält: Front Cover; Methods in Enzymology: Glycobiology; Copyright Page; Contents; Contributors; Preface; Methods in Enzymology; Section One: Proteoglycans and Sulfotransferases; Chapter One: Auxiliary and Autonomous Proteoglycan Signaling Networks; Abstract; 1. Overview of Proteoglycan Structure, Nomenclature, and Function; 2. Growth Factor Signaling; 3. Integrin Interactions; 4. Autonomous Signaling; 5. Proteoglycan Downregulation: Endocytosis and Ectodomain Shedding; 6. Cell Adhesion; 7. Migration; 8. Experimental Procedures; Acknowledgments; References
Language: English
Published: Amsterdam [u.a.], Elsevier, Acad. Press, 2010
Edition: 1. ed.
Series: Methods in enzymology (480)
Basisklassifikation: 35.77
35.76
42.15
RVK:

VK 8700.0:

WC 4355: Biologie -- Methoden -- Biochemische Methoden -- Enzymatische Analyse, Methoden der Enzymologie (auch: ELISA) -- "Methods in enzymology"

Subjects:

Glycomics

Glycosylation

Carbohydrates

Glykobiologie

Formangabe: Aufsatzsammlung
Notes: Literaturangaben
Physical Description: Online-Ressource (LII, 581 S., [8] Bl.), Ill., graph. Darst.
ISBN: 978-0-12-380999-5
0-12-381000-0
978-0-12-381000-7
Sekundärausgabe Online-Ausg., 2011

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245 1 0 |a Glycobiology  |c ed. by Minoru Fukuda 
250 |a 1. ed. 
264 1 |a Amsterdam [u.a.]  |b Elsevier, Acad. Press  |c 2010 
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490 1 |a Methods in enzymology  |v 480 
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501 |a Front Cover; Methods in Enzymology: Glycobiology; Copyright Page; Contents; Contributors; Preface; Methods in Enzymology; Section One: Proteoglycans and Sulfotransferases; Chapter One: Auxiliary and Autonomous Proteoglycan Signaling Networks; Abstract; 1. Overview of Proteoglycan Structure, Nomenclature, and Function; 2. Growth Factor Signaling; 3. Integrin Interactions; 4. Autonomous Signaling; 5. Proteoglycan Downregulation: Endocytosis and Ectodomain Shedding; 6. Cell Adhesion; 7. Migration; 8. Experimental Procedures; Acknowledgments; References 
501 |a Chapter Two: Dual Roles of Drosophila Glypican Dally-Like in Wingless/Wnt Signaling and DistributionAbstract; 1. Introduction; 2. Generation of Dally and Dlp Null Alleles; 3. Examination of Dally and Dlp in Wg Signaling and Gradient Formation in the Wing Disc; 4. Dlp Core Protein Determines Its Biphasic Activity in Wg Morphogen Signaling; 5. Dlp Core Protein Can Interact with Wg Independent of Its GAG Chains; 6. The Ratio of Dlp/Fz2 Determines the Biphasic Activity of Dlp or Dlp Core Protein in Wg Signaling; 7. Conclusion; Acknowledgments; References 
501 |a Chapter Three: Use of a Phage Display Antibody to Measure the Enzymatic Activity of the SulfsAbstract; 1. Overview; 2. ELISA for Sulf Activity Against the RB4CD12 Epitope in Immobilized Heparin/HS; 3. Flow Cytometry-Based Sulf Assay Against the Cell Surface RB4CD12 Epitope; 4. Ex Vivo Sulf Assay Against the RB4CD12 Epitope in Cryostat-Cut Sections; Acknowledgments; References; Chapter Four: Glycomics Profiling of Heparan Sulfate Structure and Activity; Abstract; 1. Overview; 2. Experimental; 3. Conclusions and Future Perspectives; Acknowledgments; References 
501 |a Section Two: Lectins, Immunity and InfectionChapter Five: Microbe-Associated Molecular Patterns in Innate Immunity; Abstract; 1. Overview; 2. LPS and LOS Extraction Procedures; 3. Purification of the Crude Extracts; 4. SDS-PAGE; 5. Carbohydrate Analysis: Monosaccharide Determination, Absolute Configuration, and Definition of Branching Points...; 6. Fatty Acids Compositional Analysis (GC-MS); References; Chapter Six: Structural and Functional Analysis of Glycosphingolipids of Schistosoma mansoni; Abstract; 1. Overview; 2. Isolation and Purification of S. mansoni Glycosphingolipids 
501 |a 3. Structural Characterization of S. mansoni Glycosphingolipids4. Immunochemical Characterization of Glycan Antigens; 5. Interaction of S. mansoni Glycosphingolipids with Dendritic Cell Receptors; 6. Conclusions; References; Chapter Seven: Biotoxicity Assays for Fruiting Body Lectins and Other Cytoplasmic Proteins; Abstract; 1. Overview; 2. Expression of Fruiting Body Lectins in E. coli; 3. Toxicity Test Toward the Insect A. aegypti; 4. Toxicity Test Toward the Nematode C. elegans; 5. Toxicity Test Toward the Amoeba A. castellanii; 6. Statistics; Acknowledgments; References 
501 |a Chapter Eight: Carbohydrate Signaling by C-Type Lectin DC-SIGN Affects NF-kappaB Activity 
501 |a Auxiliary and autonomous proteoglycan signaling networks / Arye Elfenbein and Michael SimonsDual roles of drosophila glypican dally-like in wingless/Wnt signaling and distribution / Yihui Wu, Tatyana Y. Belenkaya, and Xinhua Lin 
501 |a Use of a phage display antibody to measure the enzymatic activity of the sulfs / Kenji Uchimura ... [et al.] 
501 |a Glycomics profiling of heparan sulfate structure and activity / Jeremy E. Turnbull .. [et al.] 
501 |a Microbe-associated molecular patterns in innate immunity: extraction and chemical analysis of gram-negative bacterial lipopolysaccharides / Cristina De Castro .. [et al.] 
501 |a Structural and functional analysis of glycosphingolipids of Schistosoma mansoni / Irma van Die ... [et al.] 
501 |a Biotoxicity assays for fruiting body lectins and other cytoplasmic proteins / Markus Künzler ... [et al.] 
501 |a Carbohydrate signaling by C-type lectin DC-SIGN affects NF-kB activity / Sonja I. Gringhuis and Teunis B.H. Geijtenbeek 
501 |a Engineered carbohydrate-recognition domains for glycoproteomic analysis of cell surface glycosylation and ligands for glycan-binding receptors / Alex S. Powlesland ... [et al.] 
501 |a Mannose 6-phosphate receptor homology domain-containing lectins in mammalian endoplasmic reticulum-associated degradation / Nobuko Hosokawa, Koichi Kato, and Yukiko Kamiya 
501 |a Multiple functional targets of the immunoregulatory activity of galectin-1: control of immune cell trafficking, dendritic cell physiology, and T-cell fate / Dianne Cooper ... [et al.] 
501 |a Manipulating cell surface glycoproteins by targeting N-glycan-galectin interactions / Ani Grigorian and Michael Demetriou 
501 |a Galectin-1 and HIV-1 infection / Christian St-Pierre ... [et al.] 
501 |a The Glycomics of glycan glucuronylation in Drosophila melanogaster / Kazuhiro Aoki and Michael Tiemeyer 
501 |a Glycosyltransferases and transporters that contribute to proteoglycan synthesis in Drosophila: identification and functional analyses using the heritable and inducible RNAi system / Shoko Nishihara 
501 |a O-GlcNAc modification of the extracellular domain of notch receptors / Yuta Sakaidani, Koichi Furukawa, and Tetsuya Okajima 
501 |a Regulation of notch signaling via O-glucosylation: insights from Drosophila studies / Tom V. Lee, Hideyuki Takeuchi, and Hamed Jafar-Nejad 
501 |a O-fucosylation of thrombospondin type 1 repeats / Christina Leonhard-Melief and Robert S. Haltiwanger 
501 |a Use of glycan microarrays to explore specificity of glycan-binding proteins / David F. Smith, Xuezheng Song, and Richard D. Cummings 
501 |a Functional roles of the bisecting GlcNAc in integrin-mediated cell adhesion / Tomoya Isaji ... [et al.] 
501 |a Lectin-based glycoproteomic techniques for the enrichment and identification of potential biomarkers / Karen L. Abbott and J. Michael Pierce 
501 |a High-throughput RNAi screening for N-glycosylation dependent loci in Caenorhabditis elegans / Weston B. Struwe and Charles E. Warren 
501 |a The acidic environment of the Golgi is critical for glycosylation and transport / Yusuke Maeda and Taroh Kinoshita 
501 |a Enzymatic synthesis of lacto-N-difucohexaose I which binds to Helicobacter pylori / Tatsuo Miyazaki ... [et al.] 
501 |a The Drosophila 7-pass transmembrane glycoprotein BOSS and metabolic regulation: what Drosophila can teach us about human energy metabolism / Ayako Kohyama-Koganeya and Yoshio Hirabayashi. 
520 |a In this 3 volume collection focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers' own research efforts Each chapter has been designed so that enough scientific background will be given in each chapter for further development of methods by readers themselves. Useful for all levels of scientists starting from the last years of colleges, graduate students, postdoctoral fellows to professors and to all levels of scientists in research institutes including industry. 
520 |a In this 3 volume collection focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers' own research efforts Each chapter has been designed so that enough scientific background will be given in each chapter for further development of methods by readers themselves. Useful for all levels of scientists starting from the last years of colleges, graduate students, postdoctoral fellows to professors and to all levels of scientists in research institutes including industry 
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