schliessen

Filtern

 

Bibliotheken

Protein-Engineering von Enzymen des Hydantoinase-Prozesses / von Geoffrey A. Behrens

Carbamoylases and hydantoinases are industrially used in the hydantoinase-process for synthesis of enantiopure amino acids. Using a hydantoin-racemase enables a theoretical yield of 100%. This thesis aimed at the protein-engineering of the partaking enzymes in order to allow for new substrates. The... Full description

PPN (Catalogue-ID): 819643726
Personen: Behrens, Geoffrey A. [VerfasserIn]
Format: eBook eBook
Language: German
Published: Greifswald, Universitätsbibliothek,
Hochschule: @Greifswald, Univ., Diss., 2014
Basisklassifikation: 35.76 Aminosäuren Peptide Eiweiße
35.74 Enzyme Hormone Vitamine
58.30 Biotechnologie
Subjects:

Proteindesign

Dihydropyrimidinase

Molekulardesign

Hydantoin

Enzym

Proteindesign / Dihydropyrimidinase / Molekulardesign / Hydantoin / Enzym

Formangabe: Hochschulschrift
Physical Description: Online-Ressource (PDF-Datei: 185 S., 17385 KB), Ill., graph. Darst.

Similar Items

Vorhandene Hefte/Bände

more (+)

Informationen zur Verfügbarkeit werden geladen

Staff View
LEADER 06594nmm a2201285 c 4500
001 819643726
003 DE-601
005 20190222190914.0
007 cr uuu---uuuuu
008 150306s2014 gw o 000 0 ger d
015 |a 15O04  |2 dnb 
016 7 |a 1068088931  |2 DE-101 
024 7 |a urn:nbn:de:gbv:9-002169-2  |2 urn 
035 |a (OCoLC)906182145 
035 |a (DE-101)1068088931 
035 |a (DE-599)GBV819643726 
040 |b ger  |c GBVCP  |e rda 
041 0 |a ger 
082 0 0 |a 570 
082 0 9 |a 570 
084 |a 35.76  |9 Aminosäuren  |9 Peptide  |9 Eiweiße  |2 bkl 
084 |a 35.74  |9 Enzyme  |9 Hormone  |9 Vitamine  |2 bkl 
084 |a 58.30  |9 Biotechnologie  |2 bkl 
100 1 |a Behrens, Geoffrey A.  |e verfasserin  |4 aut 
245 1 0 |a Protein-Engineering von Enzymen des Hydantoinase-Prozesses  |h Elektronische Ressource  |c von Geoffrey A. Behrens 
260 |a Greifswald  |b Universitätsbibliothek 
300 |a Online-Ressource (PDF-Datei: 185 S., 17385 KB)  |b Ill., graph. Darst. 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
502 |a @Greifswald, Univ., Diss., 2014 
520 |a Carbamoylases and hydantoinases are industrially used in the hydantoinase-process for synthesis of enantiopure amino acids. Using a hydantoin-racemase enables a theoretical yield of 100%. This thesis aimed at the protein-engineering of the partaking enzymes in order to allow for new substrates. The carbamoylase from A. aurescens was used in a gene-shuffling experiment with the closely related beta-ureidopropionases from S. kluyveri and A. tumefaciens. Furthermore rational design based on docking experiments was performed. Based on this targeted mutations as well as focused libraries were created in the carbamoylases active site. The acceptance of ß-amino acid derivatives could be achieved. The active site of a hydantoinase from Ochrobactrum spec. was enlarged using rational design in order to achieve reactivity towards 5,5-disubstituted hydantoins. Activity was monitored used a multi-step assay system. This system was based on a growth assay relying on ammonia-starvation, an NADH-assay with glutamate-dehydrogenase as coupling enzyme and direct HPLC detection. 
520 |a Proteindesign, Dihydropyrimidinase, Molekulardesign, Hydantoinase-Prozess, Carbamoylase, Selektionsassay, hydantoinase-process, selection-assay, protein-engineering, hydantoinase 
583 |a lzar:   |k Langzeitarchivierung gewährleistet 
650 7 |0 (DE-601)156887096  |0 (DE-588)4346801-9  |a Proteindesign  |2 gnd 
650 7 |0 (DE-601)12382415X  |0 (DE-588)4309311-5  |a Dihydropyrimidinase  |2 gnd 
650 7 |0 (DE-601)104533722  |0 (DE-588)4265444-0  |a Molekulardesign  |2 gnd 
650 7 |0 (DE-601)105173185  |0 (DE-588)4200501-2  |a Hydantoin  |2 gnd 
650 7 |0 (DE-601)106338153  |0 (DE-588)4014988-2  |a Enzym  |2 gnd 
655 0 7 |0 (DE-588)4113937-9  |a Hochschulschrift  |2 gnd-content 
689 0 0 |0 (DE-601)156887096  |0 (DE-588)4346801-9  |D s  |a Proteindesign 
689 0 1 |0 (DE-601)12382415X  |0 (DE-588)4309311-5  |D s  |a Dihydropyrimidinase 
689 0 2 |0 (DE-601)104533722  |0 (DE-588)4265444-0  |D s  |a Molekulardesign 
689 0 3 |0 (DE-601)105173185  |0 (DE-588)4200501-2  |D s  |a Hydantoin 
689 0 4 |0 (DE-601)106338153  |0 (DE-588)4014988-2  |D s  |a Enzym 
689 0 |5 DE-601 
776 0 8 |i Druckausg.  |t Behrens, Geoffrey A.: Protein-Engineering von Enzymen des Hydantoinase-Prozesses  |w (DE-601)819691496 
856 4 0 |u https://epub.ub.uni-greifswald.de/rewrite/index/id/type/opus3-id/value/2169 
856 4 1 |u http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2  |3 Volltext 
856 4 1 |u http://d-nb.info/1068088931/34  |3 Volltext 
856 4 1 |u https://epub.ub.uni-greifswald.de/rewrite/index/id/type/opus3-id/value/2169  |z kostenfrei  |3 Volltext 
912 |a GBV_ILN_20 
912 |a SYSFLAG_1 
912 |a GBV_GVK 
912 |a GBV_ILN_21 
912 |a GBV_ILN_22 
912 |a GBV_ILN_23 
912 |a GBV_ILN_30 
912 |a GBV_ILN_40 
912 |a GBV_ILN_60 
912 |a GBV_ILN_69 
912 |a GBV_ILN_70 
912 |a GBV_ILN_105 
912 |a GBV_ILN_110 
912 |a GBV_ILN_132 
912 |a GBV_ILN_161 
912 |a GBV_ILN_213 
912 |a GBV_ILN_293 
912 |a GBV-ODiss 
951 |a BO 
980 |2 20  |1 01  |b 1533719152  |x 0084  |y x  |z 02-04-15 
980 |2 21  |1 01  |b 1533743150  |x 0046  |y z  |z 02-04-15 
980 |2 22  |1 01  |b 1533774560  |h SUBolrd  |x 0018  |y xu  |z 02-04-15 
980 |2 23  |1 01  |b 153379720X  |h olr-d  |x 0830  |y x  |z 02-04-15 
980 |2 30  |1 01  |b 1533812845  |x 0104  |y z  |z 02-04-15 
980 |2 40  |1 01  |b 1533828601  |x 0007  |y xsn  |z 02-04-15 
980 |2 60  |1 01  |b 1533852529  |h OLRD  |x 0705  |y z  |z 02-04-15 
980 |2 69  |1 01  |b 1529549167  |l ACQ  |x 0009  |y x  |z 11-03-15 
980 |2 70  |1 01  |b 1533891044  |x 0089  |y zdo  |z 02-04-15 
980 |2 105  |1 01  |b 1596413840  |x 0841  |y z  |z 21-01-16 
980 |2 110  |1 01  |b 1533875235  |x 3110  |y x  |z 02-04-15 
980 |2 132  |1 01  |b 1591500354  |h OLR-DISS  |x 0959  |y x  |z 19-12-15 
980 |2 161  |1 01  |b 1597692964  |h ORD  |x 0960  |y x  |z 28-01-16 
980 |2 213  |1 01  |b 1533914400  |h ORD  |x 0551  |y x  |z 02-04-15 
980 |2 293  |1 01  |b 1598223224  |h ORD  |x 3293  |y xf  |z 28-01-16 
981 |2 20  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 21  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 22  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 23  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 30  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 40  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 60  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 69  |1 01  |r https://epub.ub.uni-greifswald.de/rewrite/index/id/type/opus3-id/value/2169 
981 |2 70  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 105  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 110  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 132  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 161  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 213  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
981 |2 293  |1 01  |r http://nbn-resolving.de/urn:nbn:de:gbv:9-002169-2 
983 |2 60  |1 01  |8 10  |a ho 
983 |2 69  |1 00  |8 00  |a WD 5050 
995 |2 22  |1 01  |a SUBolrd 
995 |2 23  |1 01  |a olr-d 
995 |2 60  |1 01  |a OLRD 
995 |2 132  |1 01  |a OLR-DISS 
995 |2 161  |1 01  |a ORD 
995 |2 213  |1 01  |a ORD 
995 |2 293  |1 01  |a ORD 
998 |2 23  |1 01  |0 2015-04-02:11:04:08