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DNA repair enzymes : structure, biophysics, and mechanism / edited by Brandt F. Eichman (Vanderbilt University, Nashville, TN, Unites States)

DNA Repair Enzymes, Part B, Volume 592,is the latest volume in the Methods in Enzymologyseries and the first part of a thematic that focuses DNA Repair Enzymes.Includes contributions from leading authorities working in enzymologyFocuses on DNA repair enzymesInforms and updates on all the latest deve... Full description

PPN (Catalogue-ID): 892719745
Personen: Eichman, Brandt F. [HerausgeberIn]
Format: eBook eBook
Language: English
Published: Cambridge, MA, Academic Press, [2017]
Edition: First edition
Series: Methods in enzymology (volume 592)
Basisklassifikation: 35.74
RVK:

WC 4355: Biologie -- Methoden -- Biochemische Methoden -- Enzymatische Analyse, Methoden der Enzymologie (auch: ELISA) -- "Methods in enzymology"

Subjects:

DNS-Reparatur / Enzym

Formangabe: Aufsatzsammlung
Physical Description: 1 Online-Ressource (xx, 492 Seiten), Illustrationen, Diagramme.
ISBN: 978-0-12-812515-1
978-0-12-812516-8
978-0-12-812515-1

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245 1 0 |a DNA repair enzymes  |b structure, biophysics, and mechanism  |c edited by Brandt F. Eichman (Vanderbilt University, Nashville, TN, Unites States) 
250 |a First edition 
264 1 |a Cambridge, MA  |a San Diego, CA  |a Kidlington, Oxford  |a London  |b Academic Press  |c [2017] 
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490 1 |a Methods in enzymology  |v volume 592 
520 |a DNA Repair Enzymes, Part B, Volume 592,is the latest volume in the Methods in Enzymologyseries and the first part of a thematic that focuses DNA Repair Enzymes.Includes contributions from leading authorities working in enzymologyFocuses on DNA repair enzymesInforms and updates on all the latest developments in the field of enzymology 
520 |a Title page -- Table of Contents -- Copyright -- Contributors -- Preface -- Chapter One: MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes -- Abstract -- 1 Introduction -- 2 Entry Cloning Into MacroBac Series 11 or 438 by LIC -- 3 Building Complexes by Restriction and Ligation: MacroBac Series 11 -- 4 Building Complexes by LIC: MacroBac Series 438 -- 5 Bacmid Preparation and Virus Production -- 6 Protein Expression and Viral Stock Maintenance -- 7 Trouble Shooting -- 8 Future Considerations -- Acknowledgments -- Chapter Two: Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes -- Abstract -- 1 Introduction -- 2 Cloning -- 3 Case Studies -- 4 Nucleosome Sliding and Histone Exchange Kinetic Assays -- 5 Nucleosome Sliding by Recombinant hINO80 Chromatin Remodeling Complex -- 6 Histone Exchange by Recombinant ySWR1 Chromatin Remodeling Complex -- Chapter Three: Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR -- Abstract -- 1 Introduction -- 2 Small-Angle X-Ray Scattering -- 3 Nuclear Magnetic Resonance -- 4 Summary and Conclusion-Using SAX and NMR to Characterize Architectural Remodeling -- Chapter Four: Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair -- Abstract -- 1 Introduction -- 2 Generation of Defined Cysteine Variants -- 3 Cross-linking of Defined Cysteine Variants -- 4 Use of Single-Cysteine Variants to Study Conformational Changes in DNA Mismatch Repair -- 5 Concluding Remarks -- Acknowledgments -- Chapter Five: Expression and Structural Analyses of Human DNA Polymerase θ (POLQ) -- Abstract -- 1 Introduction -- 2 Purification and Structure Determination of Human Pol θ -- 3 Crystallization and Structure Determination of Human Pol θ -- 4 Conclusions -- Acknowledgments 
520 |a Chapter Six: Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein-Nucleic Acid Complexes -- Abstract -- 1 Introduction -- 2 Purification of RNases H2 -- 3 Biochemical Assays for RNases H2 -- 4 General Considerations in the Crystallization of Protein-Nucleic Acid Complexes -- 5 Crystallization and Structure Determination of RNases H2 -- 6 Concluding Remarks -- Acknowledgment -- Chapter Seven: DNA-PKcs, Allostery, and DNA Double-Strand Break Repair: Defining the Structure and Setting the Stage -- Abstract -- 1 Introduction -- 2 Defining the Structure of DNA-PKcs -- 3 Exploiting Multiple Se-Met Substitutions -- 4 Using Se-Met Sites to Check Sequence Registration -- 5 DNA-PKcs as a Stage for the Assembly of the Actors in NHEJ -- Acknowledgments -- Chapter Eight: Single-Particle Electron Microscopy Analysis of DNA Repair Complexes -- Abstract -- 1 Introduction -- 2 Sample Preparation -- 3 Grid Preparation -- 4 Data Collection -- 5 Data Processing -- Acknowledgments -- Chapter Nine: Using Atomic Force Microscopy to Characterize the Conformational Properties of Proteins and Protein-DNA Complexes That Carry Out DNA Repair -- Abstract -- 1 Introduction -- 2 Methods to Study DNA Repair Complexes With AFM -- 3 Image Analysis -- 4 DREEM Imaging -- 5 Complementary Techniques -- 6 Conclusions -- Acknowledgments -- Chapter Ten: Single-Molecule Methods for Nucleotide Excision Repair: Building a System to Watch Repair in Real Time -- Abstract -- 1 Introduction -- 2 Preparation of Defined Lesion Substrates for AFM and DNA Tightrope Assay -- 3 Atomic Force Microscopy -- 4 Single-Molecule DNA Tightrope Assay -- 5 Conclusions -- Acknowledgments -- Chapter Eleven: Next-Generation DNA Curtains for Single-Molecule Studies of Homologous Recombination -- Abstract -- 1 Introduction -- 2 Methods -- 3 Applications -- 4 Notes 
520 |a Acknowledgments -- Chapter Twelve: Detection of Reaction Intermediates in Mg2+-Dependent DNA Synthesis and RNA Degradation by Time-Resolved X-Ray Crystallography -- Abstract -- 1 Introduction -- 2 DNA Polymerase η -- 3 Ribonuclease H1 -- 4 Endonuclease V -- 5 X-Ray Data Processing -- 6 Applications and Future Prospects -- Acknowledgments -- Chapter Thirteen: Analyzing the Catalytic Activities and Interactions of Eukaryotic Translesion Synthesis Polymerases -- Abstract -- 1 Introduction -- 2 Purification and Structures of Nonclassical Polymerases -- 3 Catalytic Activities of Nonclassical Polymerases -- 4 Interactions of Nonclassical Polymerases -- 5 Conclusions -- Acknowledgments -- Chapter Fourteen: Kinetic Methods for Studying DNA Glycosylases Functioning in Base Excision Repair -- Abstract -- 1 Introduction -- 2 Kinetic Methods to Study Catalysis by DNA Glycosylases -- 3 Analytical Methods for Monitoring DNA Glycosylase Activity -- 4 Determining Rate Constants From Kinetics Experiments -- Acknowledgment -- Chapter Fifteen: Transient Kinetic Methods for Mechanistic Characterization of DNA Binding and Nucleotide Flipping -- Abstract -- 1 Introduction -- 2 General Considerations for Monitoring Binding -- 3 Association Kinetics -- 4 Comparison of Binding and Flipping Mechanisms for AAG and AlkA -- 5 Use of Berkeley Madonna for Evaluating Kinetic Models -- 6 Useful Equations for Kinetic Analysis -- Acknowledgments -- Chapter Sixteen: What Combined Measurements From Structures and Imaging Tell Us About DNA Damage Responses -- Abstract -- 1 Introduction -- 2 Metabolic Signaling: NAD and DDR by PARP, PARG, and AIF -- 3 DNA Double-Strand Break Responses -- 4 Taking Structural Mechanisms Into Cells by Advanced Imaging -- 5 What's Ahead -- Acknowledgments -- Author Index -- Subject Index 
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